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Key Resource Table
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Key Resource Table

Journal: Cell stem cell

Article Title: Early Stem Cell Aging in the Mature Brain

doi: 10.1016/j.stem.2021.03.018

Figure Lengend Snippet: Key Resource Table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-c-Abl antibodies Cell Signaling Technology Cat#:2862S, RRID:AB_2257757 Anti-Doublecortin (DCX), rabbit polyclonal antibodies AbcaM Cat#:ab18723, RRID:AB_732011 Anti-GFAP, rabbit polyclonal antibodies Dako Cat#:Z033401–2, RRID:AB_10013382 Anti-GFP, goat polyclonal antibodies Rockland Cat#:600–101-215, RRID:AB_218182 Anti-MCM2, mouse monoclonal antibodies BD Laboratories Cat#:610701, RRID:AB_398024 Anti-Nestin, chicken polyclonal antipeptide antibody Aves Cat#:NES, RRID:AB_2314882 Anti-Nestin, goat polyclonal antibodies R&D Cat#:AF2736, RRID:AB_416673 Anti-RFP, rabbit polyclonal antibodies Rockland Cat#:600–401-379, RRID:AB_2209751 Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma Cat#: T5648 DMSO Sigma Cat#: D2650 Imatinib Mesylate (Compound) Sigma Cat#: CDS022173 Critical Commercial Assays Neural Dissociation Kit (P) Miltenyi Biotech Cat#:130–092-628, RRID:SCR_020293 SMART-Seq v4 Ultra Low Input RNA kit for sequencing Takara Bio Cat#: 634889 Nextera XT DNA Library Preparation Kit Illumina Inc. Cat#: FC-131–1024 NextSeq 500/550 High Output Kit v2 (150 cycles) Illumina Inc. Cat#: FC-404–2002 Brain Infusion Kit 3 Alzet Cat#: 0008851 Deposited Data scRNA-seq This paper GEO: GSE168031 Experimental Models: Organisms/Strains Mouse: Nestin-CreER T2 ( Balordi and Fishell, 2007 ) N/A Mouse: Ascl1-CreER T2 The Jackson Laboratories Cat#:JAX:012882, RRID:IMSR_JAX:012882 Mouse: Nestin::CFP nuc The Jackson Laboratories Cat#:JAX:034387, RRID:IMSR_JAX:034387 Mouse: Rosa-YFP f/f The Jackson Laboratories Cat#:JAX:006148, RRID:IMSR_JAX:006148 Mouse: mT/mG f/f The Jackson Laboratories Cat#:JAX:007676, RRID:IMSR_JAX:007676 Mouse: Confetti f/f The Jackson Laboratories Cat#:JAX:013731, RRID:IMSR_JAX:013731 Mouse: C57BL/6 The Jackson Laboratories Cat#:JAX:000664, RRID:IMSR_JAX:000664 Mouse: MADM11 The Jackson Laboratories Cat#:JAX:013751, RRID:IMSR_JAX:013751 Oligonucleotides (Also see Table S4 ) Actin qPCR target sequence: 5’-GGAGAGCATAGCCCTCGTAG-3’ IDT (custom order) N/A 5’-TACCACAGGCATTGTGATGG-3’ IDT (custom order) N/A Software and Algorithms Prism 6 GraphPad RRID:SCR_002798 Adobe Illustrator Adobe.com RRID:SCR_010279 Imaris 8.0 Bitplane RRID:SCR_007370 STAR ( Dobin et al., 2013 ) RRID:SCR_015899 RSEM ( Li and Dewey, 2011 ) RRID:SCR_013027 edgeR ( Robinson et al., 2010 ) RRID:SCR_012802 topGO (Alexa A and Rahnenfuhrer J, 2016) RRID:SCR_014798 limma ( Ritchie et al., 2015 ) RRID:SCR_010943 RNA velocity ( La Manno et al., 2018 ) RRID:SCR_018167 Other Osmotic pump Alzet Cat#: 1007D Open in a separate window Key Resource Table.

Techniques: Bioprocessing, Recombinant, Sequencing, DNA Library Preparation, Software